ReproSource Fertility Diagnostics
200 Forest Street, 2nd Floor, Suite B, Marlborough, MA 01752 USA
Tel: 800.667.8893 (USA only) | Fax: 781.935.3068
CLIA#: 22D0884531
Patient Information
Field
Value
Patient
Lu, Yanxin
Gender
M
Age
35Y
Date of Birth
10/17/1989
Specimen
00598276
Reported
08/26/2025
Received
08/20/2025, Time: 09:50
Collected
08/18/2025, Time: 15:15
Clinician
Field
Value
Clinician
Philip Werthman MD
Phone
1-310-277-2873
Fax
1-310-286-2139
Address
Center For Male Reproductive Medicine, 2080 Century Park East, Suite 907, Los Angeles, CA 90067
Overview
Test
Value
Range: Normal
Range: Borderline
Range: Abnormal
Result
DFI (DNA Fragmentation Index)
3
0–20
20–30
>30 (60+ severe)
Normal — DNA integrity
OSA (Oxidative Stress Adduct)
1.4
0–3.8
3.8–4.4
>4.4 (9+ severe)
Normal — Damage from free radicals/reactive oxygen species (ROS)
HDS (High DNA Stainability)
6
0–15
—
>15 (30+ severe)
Normal — Levels of decondensed DNA (eg immature sperm)
Patient Results & Interpretation
Test Name
Normal Range
Unit
Result
Interpretation
DFI — DNA Fragmentation Index
<20
%
3
Normal. Predicted success: Natural=normal, IUI=normal, IVF=normal, ICSI=normal. Higher DFI scores correlate to lower success rates in natural or IUI attempts at pregnancy. Abnormal DFI results suggest the consideration of advancing directly to IVF or ICSI, treatments that lower the DFI score, and/or consultation with a urologist specializing in fertility.
Oxidative Stress Adduct
<3.8
uM
1.4
Normal. The OSA test directly measures sperm damage from oxidative stress by quantifying the presence of "adducts," molecules in semen covalently modified by free radicals/reactive oxygen species. Men from 955 infertile couples demonstrated significantly higher results compared with 20 fertile controls (Fig 1, p<0.05). Low results have unclear clinical significance at this time.
HDS — High DNA Stainability
<15
%
6
Normal. The HDS Score provides supplementary information regarding the percent of cells with highly-staining DNA, and can be abnormal when high levels of immature sperm cells are present.
References
Tirado E, Marquette M, Musto JD, Leader B. The association of aging, oxidative stress and DNA integrity in human spermatozoa. American Society of Andrology 2010.
Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science. 1980;210(4474):1131-1133.
Bungum M, Humaidan P, Axmon A, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod. 2007;22(1):174-179.
Evenson DP, Jost LK, Marshall D, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14(4):1039-1049.
Spanò M, Bonde JP, Hjøllund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. Fertil Steril. 2000;73(1):43-50.
Erenpreiss J, Bungum M, Spano M, Elzanaty S, Orbidans S, Giwercman A. Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications. Hum Reprod. 2006;21(8):2061-2064.
Laboratory Director (CLIA): Vivekananda Datta, M.D., Ph.D.
Page 2: Werthman Semen Analysis Worksheet
Center for Male Reproductive Medicine
Lab Director: Philip Werthman, M.D., P.C.
2080 Century Park East, Suite 907, Los Angeles, CA 90067
(310) 277-2873 | Fax (310) 286-2139
