--- type: lab-results category: medical person: Yanxin Lu date: 2025-08-18 provider: ReproSource Fertility Diagnostics / Center for Male Reproductive Medicine clinician: Philip Werthman, MD source: lu_SA_DFI_08_2025.pdf --- # Yanxin Lu — ReproSource Advanced Semen Report & Werthman Semen Analysis (08/2025) ## Page 1: ReproSource Advanced Semen Report **ReproSource Fertility Diagnostics** 200 Forest Street, 2nd Floor, Suite B, Marlborough, MA 01752 USA Tel: 800.667.8893 (USA only) | Fax: 781.935.3068 CLIA#: 22D0884531 ### Patient Information | Field | Value | |-------|-------| | Patient | Lu, Yanxin | | Gender | M | | Age | 35Y | | Date of Birth | 10/17/1989 | | Specimen | 00598276 | | Reported | 08/26/2025 | | Received | 08/20/2025, Time: 09:50 | | Collected | 08/18/2025, Time: 15:15 | ### Clinician | Field | Value | |-------|-------| | Clinician | Philip Werthman MD | | Phone | 1-310-277-2873 | | Fax | 1-310-286-2139 | | Address | Center For Male Reproductive Medicine, 2080 Century Park East, Suite 907, Los Angeles, CA 90067 | ### Overview | Test | Value | Range: Normal | Range: Borderline | Range: Abnormal | Result | |------|-------|--------------|-------------------|----------------|--------| | DFI (DNA Fragmentation Index) | **3** | 0–20 | 20–30 | >30 (60+ severe) | **Normal** — DNA integrity | | OSA (Oxidative Stress Adduct) | **1.4** | 0–3.8 | 3.8–4.4 | >4.4 (9+ severe) | **Normal** — Damage from free radicals/reactive oxygen species (ROS) | | HDS (High DNA Stainability) | **6** | 0–15 | — | >15 (30+ severe) | **Normal** — Levels of decondensed DNA (eg immature sperm) | ### Patient Results & Interpretation | Test Name | Normal Range | Unit | Result | Interpretation | |-----------|-------------|------|--------|---------------| | DFI — DNA Fragmentation Index | <20 | % | **3** | **Normal**. Predicted success: Natural=normal, IUI=normal, IVF=normal, ICSI=normal. Higher DFI scores correlate to lower success rates in natural or IUI attempts at pregnancy. Abnormal DFI results suggest the consideration of advancing directly to IVF or ICSI, treatments that lower the DFI score, and/or consultation with a urologist specializing in fertility. | | Oxidative Stress Adduct | <3.8 | uM | **1.4** | **Normal**. The OSA test directly measures sperm damage from oxidative stress by quantifying the presence of "adducts," molecules in semen covalently modified by free radicals/reactive oxygen species. Men from 955 infertile couples demonstrated significantly higher results compared with 20 fertile controls (Fig 1, p<0.05). Low results have unclear clinical significance at this time. | | HDS — High DNA Stainability | <15 | % | **6** | **Normal**. The HDS Score provides supplementary information regarding the percent of cells with highly-staining DNA, and can be abnormal when high levels of immature sperm cells are present. | ### References 1. Tirado E, Marquette M, Musto JD, Leader B. The association of aging, oxidative stress and DNA integrity in human spermatozoa. *American Society of Andrology 2010*. 2. Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. *Science*. 1980;210(4474):1131-1133. 3. Bungum M, Humaidan P, Axmon A, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. *Hum Reprod*. 2007;22(1):174-179. 4. Evenson DP, Jost LK, Marshall D, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. *Hum Reprod*. 1999;14(4):1039-1049. 5. Spanò M, Bonde JP, Hjøllund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. *Fertil Steril*. 2000;73(1):43-50. 6. Erenpreiss J, Bungum M, Spano M, Elzanaty S, Orbidans S, Giwercman A. Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications. *Hum Reprod*. 2006;21(8):2061-2064. **Laboratory Director (CLIA)**: Vivekananda Datta, M.D., Ph.D. --- ## Page 2: Werthman Semen Analysis Worksheet **Center for Male Reproductive Medicine** Lab Director: Philip Werthman, M.D., P.C. 2080 Century Park East, Suite 907, Los Angeles, CA 90067 (310) 277-2873 | Fax (310) 286-2139 ### Specimen Information | Field | Value | |-------|-------| | Patient Name | Yanxin Lu | | DOB | SA 08/18/25 (handwritten) | | Date of Specimen | 8/18/25 | | Time Collected | 3:12 | | Age of Specimen | 50 min | | Days Since Last Ejaculation | 2 | | Any Portion Lost? | No | ### Specimen Purpose - [x] Complete Semen Analysis - [ ] Count only - [ ] Insemination prep - [x] DFI/OSA - [ ] Culture and sensitivity - [ ] Freeze ### Patient Results | Parameter | Value | Normal Range (WHO, 2006) | Normal Range (WHO, 2010) | |-----------|-------|------------------------|------------------------| | Volume (cc) | 5.L (~5) | 2.0–5.0 cc | 1.5–6 cc | | Count (mil/cc) | h (~high/normal) | >20 mil/cc | >15 mil/cc | | Motility (%) | 56% | >50% | >32% | | Grade | 2+/3 | 3–4 | 3–4 | | pH | 8.0 | 7.2–8.0 | ≥7.2 | | Round Cells (lpf) | 0–1 | <10/lpf | <1 | | Viscosity | N (Normal) | Normal | Normal | | Agglutination | d (none) | None | None | | Normal Morphology (%) | 3 | >30% | >4% (strict criteria) | | Head Defects (%) | — | — | — | | Midpiece (%) | — | — | — | | Tail (%) | — | — | — | | Total Abnormal (%) | — | — | — | ### Comments **Progyny** (handwritten comment)